Asymmetric somatic hybrid plants between an interspecific Lycopersicon hybrid and Solanum melongena.

TitleAsymmetric somatic hybrid plants between an interspecific Lycopersicon hybrid and Solanum melongena.
Publication TypeJournal Article
Year of Publication1995
AuthorsLiu KB, Li YM, Sink KC
JournalPlant cell reports
Volume14
Issue10
Pagination652-6
Date Published1995 Jul
Abstract

Asymmetric somatic hybrid plants were obtained by a modified PEG/DMSO fusion procedure between protoplasts derived from suspension cells of an interspecific tomato hybrid, Lycopersicon esculentum x L. pennellii, and mesophyll protoplasts of Solanum melongena, eggplant. The tomato hybrid was previously transformed with Agrobacterium tumefaciens and contained the kanamycin-resistance marker gene. Prior to fusion, the donor protoplasts of the tomato hybrid were gamma irradiated at 9.0 krad. Thus, non-division of irradiated tomato hybrid protoplasts coupled with kanamycin sensitivity of eggplant enabled selection of somatic cell hybrids. Forty-nine calli selected post-fusion regenerated leaf-like structures in the presence of 50 mg/l kanamycin. However, only four of the 49 calli regenerated intact shoots which rooted in the presence of 50 mg/l kanamycin and were later transferred to the greenhouse. Analysis of phosphoglucoisomerase and peroxidase isozymes, and Southern hybridization with a nuclear-specific pea 45 S ribosomal RNA gene confirmed somatic hybrid status. Cytology revealed that the four hybrid plants had chromosome numbers of 45, 60, 42 and 57, respectively; they were all sterile.

DOI10.1155/2013/952350
Alternate JournalPlant Cell Rep.