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Double-stranded regions in denatured DNA from mouse cells.
|Title||Double-stranded regions in denatured DNA from mouse cells.|
|Publication Type||Journal Article|
|Year of Publication||1973|
|Authors||Church RB, Georgiev GP|
|Journal||Molecular biology reports|
|Date Published||1973 Jun|
Approximately 2% of the DNA of the mouse genome reassociates at infinitely low C 0 t values, 10(-7) to 10(-6) moles 1(-1) s. The melting profile of the reassociation product, which is resistant to nuclease S1 digestion, has been characterized by hydroxyapatite column chromatography. The properties of these nuclease resistant sequences suggest that they exist as 'DNA-hairpins' and that they originate from reverted base sequences within the genome.
|Alternate Journal||Mol. Biol. Rep.|