Fast and efficient molecule detection in localization-based super-resolution microscopy by parallel adaptive histogram equalization.

TitleFast and efficient molecule detection in localization-based super-resolution microscopy by parallel adaptive histogram equalization.
Publication TypeJournal Article
Year of Publication2013
AuthorsLi Y, Ishitsuka Y, Hedde PN, Nienhaus UG
JournalACS nano
Volume7
Issue6
Pagination5207-14
Date Published2013 Jun 25
Abstract

In localization-based super-resolution microscopy, individual fluorescent markers are stochastically photoactivated and subsequently localized within a series of camera frames, yielding a final image with a resolution far beyond the diffraction limit. Yet, before localization can be performed, the subregions within the frames where the individual molecules are present have to be identified-oftentimes in the presence of high background. In this work, we address the importance of reliable molecule identification for the quality of the final reconstructed super-resolution image. We present a fast and robust algorithm (a-livePALM) that vastly improves the molecule detection efficiency while minimizing false assignments that can lead to image artifacts.

DOI10.1107/S1600536813013056
Alternate JournalACS Nano