FLUORESCENCE KINETICS OF COMPLEX FORMATION BETWEEN HUMAN SERUM ALBUMIN AND ZINC-PHTHALOCYANINE TETRASULFONIC ACID.

TitleFLUORESCENCE KINETICS OF COMPLEX FORMATION BETWEEN HUMAN SERUM ALBUMIN AND ZINC-PHTHALOCYANINE TETRASULFONIC ACID.
Publication TypeJournal Article
Year of Publication2011
AuthorsJones CL, Fields A, Thomas L, Patel S, Biggins S
JournalJournal of undergraduate chemistry research
Volume10
Issue1
Pagination9-11
Date Published2011 Winter
Abstract

Stopped-flow fluorescence was employed to measure the dissociation constant, activation energy, and frequency factor for zinc phthalocyanine tetrasulfonic acid binding to human serum albumin. A pseudo 1(st) order "Isolation Method" was used to measure temperature dependent rate constants at pH 7.00. Binding was followed by a relatively large emission signal that indicated the formation of a protein-ligand complex. The dissociation constant, activation energy and frequency factor were 3.3 (±0.3)×10(-5) (M), 4.5 (±0.2)×10(4) J/mol, and 1.1 (±0.1)×10(8) min(-1) respectively.

DOI10.1371/journal.pone.0074635
Alternate JournalJ Undergrad Chem Res