Generation of transgenic rats through induced pluripotent stem cells.

TitleGeneration of transgenic rats through induced pluripotent stem cells.
Publication TypeJournal Article
Year of Publication2013
AuthorsJiang M-G, Li T, Feng C, Fu R, Yuan Y, Zhou Q, Li X, Wan H, Wang L, Li W, Xiao Y, Zhao X-Y, Zhou Q
JournalThe Journal of biological chemistry
Volume288
Issue38
Pagination27150-8
Date Published2013 Sep 20
Abstract

The rat is an important animal model for human disease research. Using inhibitors of glycogen synthase kinase 3 and MAPK signaling pathways, rat embryonic stem cells and rat induced pluripotent stem cells (riPSCs) have been derived. However, unlike rat embryonic stem cells, germ line competent riPSCs have only been derived from Wistar rats at low efficiency. Here, we found that an optimized induction medium containing knock-out serum replacement and vitamin C improved the rate and efficiency of riPSCs generation from Dark Agouti rat fibroblasts and Sertoli cells. riPSCs maintained an undifferentiated status for >30 passages and could differentiate into various cells types including germ cells when injected into rat blastocysts. Moreover, transgenic riPSCs could be generated through the PiggyBac transposon, which could be used to generate transgenic rats through germ line transmission. riPSCs can be used as a novel tool in genetic and genomic studies of the rat.

DOI10.2147/NDT.S47099
Alternate JournalJ. Biol. Chem.