Identification of widespread adenosine nucleotide binding in Mycobacterium tuberculosis.

TitleIdentification of widespread adenosine nucleotide binding in Mycobacterium tuberculosis.
Publication TypeJournal Article
Year of Publication2013
AuthorsAnsong C, Ortega C, Payne SH, Haft DH, Chauvignè-Hines LM, Lewis MP, Ollodart AR, Purvine SO, Shukla AK, Fortuin S, Smith RD, Adkins JN, Grundner C, Wright AT
JournalChemistry & biology
Volume20
Issue1
Pagination123-33
Date Published2013 Jan 24
Abstract

Computational prediction of protein function is frequently error-prone and incomplete. In Mycobacterium tuberculosis (Mtb), ~25% of all genes have no predicted function and are annotated as hypothetical proteins, severely limiting our understanding of Mtb pathogenicity. Here, we utilize a high-throughput quantitative activity-based protein profiling (ABPP) platform to probe, annotate, and validate ATP-binding proteins in Mtb. We experimentally validate prior in silico predictions of >240 proteins and identify 72 hypothetical proteins as ATP binders. ATP interacts with proteins with diverse and unrelated sequences, providing an expanded view of adenosine nucleotide binding in Mtb. Several hypothetical ATP binders are essential or taxonomically limited, suggesting specialized functions in mycobacterial physiology and pathogenicity.

DOI10.1167/iovs.12-10847
Alternate JournalChem. Biol.