Road Closures Near 801 Broadway Parking Garage
News & Updates
Search Research Content
Resource Finder at Kennedy Krieger Institute
A free resource that provides access to information and support for individuals and families living with developmental disabilities.
Indirect detection of labile solute proton spectra via the water signal using frequency-labeled exchange (FLEX) transfer.
|Title||Indirect detection of labile solute proton spectra via the water signal using frequency-labeled exchange (FLEX) transfer.|
|Publication Type||Journal Article|
|Year of Publication||2010|
|Authors||Friedman JI, McMahon MT, Stivers JT, van Zijl PCM|
|Journal||Journal of the American Chemical Society|
|Date Published||2010 Feb 17|
We report an "exchange-rate-filtered" magnetic resonance approach that allows the detection of exchangeable protons of low-concentration solutes without interference from nonexchanging protons. This indirect detection of signals of multiple rapidly exchanging protons through the water signal can be achieved while retaining chemical shift specificity and increasing the sensitivity by several orders of magnitude with respect to standard spectroscopy. This frequency-labeled exchange (FLEX) transfer principle is applied to detect previously "invisible" protons of some nucleic acids and peptides as well as rapidly exchanging protons (k > 300 s(-1)) in so-called chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI) contrast agents. The FLEX methodology is expected to provide a practical approach for the study of highly dynamic regions of nucleic acids and proteins where amide, amino, and imino groups are rapidly moving between a closed solvent-inaccessible state and an exposed state where exchange occurs. This alternative method of labeling and detecting exchangeable protons is also expected to greatly benefit the development of new exchange-based MRI contrast agents, providing a method for multifrequency detection using frequency transfer instead of saturation transfer.
|Alternate Journal||J. Am. Chem. Soc.|