News & Updates
Search Research Content
Resource Finder at Kennedy Krieger Institute
A free resource that provides access to information and support for individuals and families living with developmental disabilities.
A method for assessment of blood volume parameters in pregnant sheep using fluorescein-labelled dextran.
|Title||A method for assessment of blood volume parameters in pregnant sheep using fluorescein-labelled dextran.|
|Publication Type||Journal Article|
|Year of Publication||2008|
|Authors||Rumball CWH, van Zijl P, Rutland MD, Bloomfield FH, Harding JE|
|Date Published||2008 Jan|
The assessment of blood volume parameters in clinical and research settings has been limited by methods that involve radioactivity, complex assays or are unreliable. We aimed to design a method for measuring blood volume parameters that was non-radioactive, simple, cheap and reliable. We have used a commercially available fluorescein-labelled 250kDa dextran, a large inert molecule, and have measured dilution of this through the intravascular space of pregnant ewes. From this estimation of plasma volume and measured hematocrit, we have calculated blood volume and red cell volume. The blood volume results are 6% lower than those obtained using radiolabelled red cells, but there is no significant difference in red cell volume between methods. The coefficient of variation for repeated measurements of plasma volume measurements is 3.8%. This is a simple, reliable, cheap and non-radioactive method for estimating blood volume parameters in pregnant sheep, and may prove useful in other settings.