Stem cell profiling by nuclear magnetic resonance spectroscopy.

TitleStem cell profiling by nuclear magnetic resonance spectroscopy.
Publication TypeJournal Article
Year of Publication2006
AuthorsJansen JFA, Shamblott MJ, van Zijl PCM, Lehtimäki KK, Bulte JWM, Gearhart JD, Hakumäki JM
JournalMagnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine
Volume56
Issue3
Pagination666-70
Date Published2006 Sep
Abstract

The classification of embryonic and adult stem cells, including their derivatives, is still limited, and often these cells are best defined by their functional properties. Recent gene array studies have yielded contradictory results. Also, very little is known about the metabolic properties of these exciting cells. In this study, proton (1H) NMR spectroscopy was used to identify the major low-molecular-weight metabolites in murine embryonic stem cells (ESC) and their neural stem cell (NSC) derivatives. ESC are characterized by an unusually low number of NMR-detectable metabolites, high phosphocholine (PC) content, and nondetectable glycerophosphocholine (GPC). The metabolic profiles of NSC resemble glial cells and oligodendrocyte progenitors, but with considerably higher PC, GPC, and myo-inositol content. The results suggest that NMR spectroscopy in vitro can provide markers to study the effects of differentiation on cell metabolism, and potentially to assess stem cell preparations for differentiation status.

Alternate JournalMagn Reson Med