Telomere position effect regulates DUX4 in human facioscapulohumeral muscular dystrophy.

TitleTelomere position effect regulates DUX4 in human facioscapulohumeral muscular dystrophy.
Publication TypeJournal Article
Year of Publication2013
AuthorsStadler G, Rahimov F, King OD, Chen JCJ, Robin JD, Wagner KR, Shay JW, Emerson CP, Wright WE
JournalNature structural & molecular biology
Volume20
Issue6
Pagination671-8
Date Published2013 Jun
Abstract

Telomeres may regulate human disease by at least two independent mechanisms. First, replicative senescence occurs once short telomeres generate DNA-damage signals that produce a barrier to tumor progression. Second, telomere position effects (TPE) could change gene expression at intermediate telomere lengths in cultured human cells. Here we report that telomere length may contribute to the pathogenesis of facioscapulohumeral muscular dystrophy (FSHD). FSHD is a late-onset disease genetically residing only 25-60 kilobases from the end of chromosome 4q. We used a floxable telomerase to generate isogenic clones with different telomere lengths from affected patients and their unaffected siblings. DUX4, the primary candidate for FSHD pathogenesis, is upregulated over ten-fold in FSHD myoblasts and myotubes with short telomeres, and its expression is inversely proportional to telomere length. FSHD may be the first known human disease in which TPE contributes to age-related phenotype.

DOI10.1039/c3an01532g
Alternate JournalNat. Struct. Mol. Biol.